The full-back has been out of action since sustaining a medial knee ligament injury in a goalless draw against Sunderland at the end of January.
Rose had returned to training with Spurs, but has now required further treatment and reports suggest that the 26-year-old will miss the start of next season.
A club statement read: “The England international sustained the injury in January against Sunderland.
“He recently returned to first-team training, however the problem has not resolved fully therefore a decision has been made to proceed to surgery.
“He will now return to action next season.”
Rose has made 18 Premier League appearances this term, scoring two goals.
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